5.

Effects of mito-K_ATP channel opener diazoxide on rotenone-induced dopaminer-gic neuronal death and microglial activation in rat substantia nigra. (A) Images of immunohistochemistry. TH-positive cells (brown) represent dopaminergic neurons. Anti-GFAP was employed for staining astro-cytes (brown). OX-42 and ED1 immunos-taining (brown) were carried out to study microglial activation. Scale bar: 100μm (black), 20 μm (white). (B) TH-positive cell numbers in substantia nigra. ***P<0.001 versus control group; ###P <0.001 versus rotenone (2.5 mg/kg/day) group. (C) GFAP-positive cell numbers in substantia nigra. Data are expressed as mean ± S.E.M., n = 6 per group.