2.

Adhesion of CXCR6-HEK293 cells to homocysteine-stimulated endothelial cells. (A) HEK293 cells were transfected to stably express CXCR6 as shown by flow cytometry. (B) As determined by the fluorescence signal of adherent CXCR6-HEK293 cells, Hcy but not l-Cys significantly increased the adhesiveness of EC (n = 5 experiments; *P < 0.05 versus control). Pre-treatment of Hcy-stimu-lated EC with a neutralizing antibody against CXCL16 diminished Hcy's stimulatory effect ($P < 0.05 versusl-Hcy only). In (C) representative microscopy pictures showing adhesion of CXCR6-HEK293 cells to unstim-ulated and Hcy-stimulated EC are presented.