4.

Hcy's stimulatory effect on uptake of DiI-labelled oxLDL by endothelial cells. (A) EA.hy926 cells were assayed for binding of DiI-oxLDL by flow cytometry. (B) Binding of DiI-oxLDL to EC was significantly increased by 24 hrs stimulation of EC with Hcy but not by treatment with l-Cys (n = 4 experiments; *P <0.05 versus untreated control cells). Pre-incubation of Hcy-activated ECs with neutralizing antibody against CXCL16 significantly dimished the uptake of oxLDL (^$P <0.05 versusl-Hcy only). (C) Representative photomicrographs indicate increased uptake of oxLDL by Hcy-treated EC.