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Sp3 inhibits TβRII transcription through the Sp1 site at −25 of human TβRII promoter. A: HAC were nucleofected with 2 μg of pCMV-Sp3 (or insertless pCMV vector), and TβRII promoter constructs. After 24 hrs, luciferase activities (RLU) were determined and expressed as per cent of controls. B: Human chondro-cytes were nucleofected with siRNA targeted Sp1 and/or Sp3, and TβRII mRNA levels were analysed by real-time RT-PCR after 24 hrs of incubation.
