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NFκB p65 subunit inhibits TβRII transcription through the Sp1 site at −25 of human TβRII promoter. HAC were nucleofected with 2 μg of pSG5-p65 (or insertless pSG5 vector), and wild-type (A) or mutated (B) TβRII promoter constructs. After 24 hrs, luciferase activities were determined and expressed as RLU.
