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. 2013 Jan 15;6(5):503–514. doi: 10.1111/1751-7915.12031

Figure 1.

Figure 1

Schematic organization of the ArsR-controlled genetic circuits assembled on plasmids in E. coli.A. Elements building the feedback arsR-egfp construct.B. As (A), but with the arsR–mCherry fusion gene.C. The uncoupled arsenic bioreporter circuits.D. The tetR gene under control of the lac promoter. E. Uncoupled circuit with the arsR–mCherry fusion gene. The position of the binding site for ArsR on the DNA is depicted by dark vertical bars (ABS); those for TetR by grey vertical bars. Positions of restriction sites relevant for cloning are indicated. Outline of (C) indicative for plasmids pAAUN, pLtetOUN, pIIUN, pKUN, pVUN and pJJUN. Those in (E) for pAAUNmChe and pLtetOUNmChe.