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. 2013 Feb 28;6(5):551–563. doi: 10.1111/1751-7915.12040

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© 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Expression of pha genes and PHA accumulation in P. putida U. Each panel shows normalized fold-increased in expression of the pha genes in PpU (violet), PpU 10–33 non induced (light blue) and PpU 10–33 induced (dark blue), PpU10-33-ΔphaZ uninduced (light green) and PpU10-33-ΔphaZ induced cells (dark green). The relative expression ratio of the target genes was calculated automatically with the CFX software using the standard error of the mean and the normalized expression method [ΔΔ(Ct)]. PHA content (% CDW) is showed with lines, using the same strain colour designation mentioned above. Experimental triplicates and independent biological duplicates were used for the calculations.