Figure 2.

Kinetic parameters of wild-type small laccase (SLAC) and the S292A variant enzyme. Kinetic parameters were determined for wild-type (wt) SLAC on ABTS, 2,6-DMP, quercetin, morin and myricetin. Kinetic parameters were also determined for S292A on ABTS, 2,6-DMP, and morin, but could not be obtained for quercetin and myricetin since the apparent K_m of the enzyme for these substrates exceeded substrate solubility. Lighter shaded regions in quercetin, morin and myricetin molecules indicate identical chemical structures. n = 3; errors indicate standard deviation.