Table 1. Primers Sequence Used for Amplification cdd3, tcdB and tcdA Genes .
Gene | Primer | Nucleotide sequence | Fragment Length (bp) |
---|---|---|---|
Cdd-3 | Tim6 | 5´ TCC AAT ATA ATA AAT TAG CAT TCC A 3´ | 622 |
Struppi6 | 5´ GGC TAT TAC ACG TAA TCC AGA TA 3´ | ||
TcdA | TA1 | 5´ATG ATA AGG CAA CTT CAG TGG 3´ | 624 |
TA2 | 5´TAA GTT CCT CCT GCT CCA TCA A 3´ | ||
TcdB | TB1 | 5´GAG CTG CTT CAA TTG GAG AGA 3´ | 412 |
TB2 | 5´GTA ACC TAC TTT CAT AAC ACC AG 3´ |