Lentivirus construct used to transduce schwannoma cells with luciferase gene. Essential viral cis elements for vector infection: LTRs indicate long terminal repeats; RRE, rev-responsive element; ψ (packaging signal); SIN, self-inactivated vector; cPPT, HIV central polypurine tract or central flap; WPRE, woodchuck hepatitis virus posttranscriptional regulatory element; SFFV promoter, spleen focus forming virus—has strong promoter activity in most human or mouse cells. BamHI and NotI are restriction endonuclease sites.