Fig. 1.

Distribution of CD1a⁺ dendritic cells within airway mucosa of HDM-challenged rhesus monkeys. CD1a⁺ dendritic cells were identified by immunofluorescence staining of trachea and left caudal lobe sections obtained from HDM-challenged monkeys. Tr, P1, P2, M1, M2, and RB represent cryosections obtained from tissue blocks of trachea and regions progressively representing the most proximal (P1) to distal (RB) intrapulmonary airways of the lobe. Columns represent the average volume ± SE of CD1a⁺ staining cells (mm³) with respect to the surface area of basal lamina (mm²). FA, filtered air control; HDM, house dust mite-challenged; RB, respiratory bronchiole. (a) CD1a⁺ immunofluorescence staining of trachea from a representative HDM monkey. Bar=20 μm. (b) Control staining of an adjacent section using an FITC-conjugated o mouse IgG1 isotype control. Bar=20 μm. (c) Abundance of CD1a⁺ cells within the epithelial compartment of conducting airways. ND, none detected; *P<0.0001 by analysis of variance (block effect); **P=0.0005 by analysis of variance (treatment effect). (d) Abundance of CD1a⁺ cells within the interstitial compartment of conducting airways. *P=0.0284 by analysis of variance (treatment effect).