Fig. 6.

Cytokine and chemokine gene expression within airway mucosa of house dust mite (HDM)-challenged rhesus monkeys. The relative mRNA expression of the listed cytokines, chemokines, and chemokine receptors were evaluated from trachea and microdissected intrapulmonary airways obtained from the right caudal lobe of HDM-challenged monkeys. Samples were collected from the trachea, most proximal intrapulmonary airway, a midlevel airway, and RB for RNA isolation; separately collected samples from each airway generation were processed for histology. (a) H&E staining of microdissected samples, showing both epithelial and interstitial organization of each airway generation. (b) Relative mRNA expression profile for different airway generations in three (#1, #2, #3) different HDM-challenged monkeys. Relative gene expression for each cytokine, chemokine, or chemokine receptor was determined from averaged Ct values from a pool of five control monkeys.