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. Author manuscript; available in PMC: 2014 Feb 9.
Published in final edited form as: Nat Cell Biol. 2011 Feb 20;13(3):292–302. doi: 10.1038/ncb2170

Figure 4. E2F target genes are not repressed by heterochromatin induction in DDR-deficient oncogene-expressing cells.

Figure 4

(a) qRT–PCR analyses show that, despite increased global heterochromatin, E2F-target genes (MCM6,PCNA and CCNA2) are not repressed in DDR-deficient oncogene-expressing cells. mRNA levels were normalized to the levels in cells transduced with empty vector. (b) Quantitative ChIP results for H3K9me3 from the promoter regions of three E2F-target genes analysed in a. H3K9me3 levels are expressed as a percentage of the non-immunoprecipitated DNA (input), normalized to the percentage of the total immunoprecipitated histone H3 (to correct for nucleosome variations) and further normalized to the levels in cells transduced with empty vector. These results are representative of three independent experiments.