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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Cell Rep. 2013 Dec 5;5(5):1375–1386. doi: 10.1016/j.celrep.2013.11.007

Figure 4.

Figure 4

2-APB induces egg activation in TrpV3+/− eggs but not in TrpV3−/− MII eggs. A. Treatment with 2-APB (200 μM; 37°C) for 30 min activates eggs. Left panel: 2-APB induces cleavage, observed 24 h after 200 μM 2-APB treatment in TrpV3-Het MII eggs, but not in TrpV3-KO eggs. Right panel: Percentage of eggs undergoing cleavage at 24 h. Numbers of 2, 3, or 4-cell blastomeres over the total number of eggs is indicated. Data are averages ± S.E.M. B. Ionomycin (2.5 μM for 5 min) at 37°C induces activation of TrpV3-KO eggs. Numbers of eggs with PN formation/total number of eggs is indicated. Arrows indicate PN formation (right panel).