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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Cell Rep. 2013 Dec 5;5(5):1375–1386. doi: 10.1016/j.celrep.2013.11.007

Figure 6.

Figure 6

Calcium influx through TRPV3 channels does not contribute to early development in mouse eggs. A. Oscillations induced by sperm fertilization in heterozygous (left, TrpV3+/−, green line, n=12/24 have 5–6 oscillations in 60 min) and V3-KO (right) eggs (blue line, n=9/18 have 5–6 oscillations in 60 min). B. In vitro fertilization (IVF) rates measured by 2-cell formation after 24 h of fertilization showed no difference between V3-Het and V3-KO eggs (71 ± 12 for V3-Het vs. 80 ± 4 for V3-KO eggs, p > 0.05). Numbers of 2-cell blastomeres/total number of eggs is indicated. Data are averages ± S.E.M. C. [Ca2+]i responses were induced by injection of 0.01μg/μl mPLCζ cRNA (similar responses were obtained by 0.05 μg/μl mPLCζ cRNA injection). [Ca2+]i oscillations (37°C) in a control egg (TrpV3+/−, left panel, n=8) and KO egg (TrpV3−/−, right panel, n=3). 200 μM 2-APB (red bar) was applied at the end of the experiment.