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. Author manuscript; available in PMC: 2014 Jun 12.
Published in final edited form as: Mol Cell. 2013 Nov 14;52(5):617–628. doi: 10.1016/j.molcel.2013.10.014

Fig. 1. Mutations in the Toxin socB Bypass ClpXP Essentiality.

Fig. 1

(A) Schematic of transposon insertions in socB (CCNA_03629) that suppressed the essentiality of clpP.

(B) Growth of clpX and clpP depletion strains in socB+ and ΔsocB backgrounds. Five-fold serial dilutions of the indicated strains were spotted onto media ± IPTG.

(C) Kinetics of ClpX depletion. Indicated strains were shifted to media ± IPTG, and samples were subjected to immunoblotting.

(D) Morphology of cells following ClpX depletion in socB+ and ΔsocB backgrounds. Strains from (C) were imaged by DIC microscopy at 10 hr.

(E) Viability of cells following ClpX depletion in socB+ and ΔsocB backgrounds. Colony forming units (CFUs)/ml of the strains from (C) are shown; mean of two biological replicates.

(F) Growth of strains expressing socB in the socA+ or ΔsocA backgrounds. The indicated strains were five-fold serially diluted onto media that induces or represses socB.

(G) Morphology of strains from (F). The indicated strains were grown for 4 hr in socB inducing conditions and then imaged by DIC microscopy.

(H) Bacterial two-hybrid analysis of the interaction between SocA and SocB. T18/T25 were included as a negative control; red indicates a positive interaction. Cells were grown for 1 day at 30°C.

See also Fig. S1.