. Author manuscript; available in PMC: 2014 Dec 20.

Published in final edited form as: ACS Chem Biol. 2013 Nov 14;8(12):2607–2621. doi: 10.1021/cb400689r

Table 2.

Effect of metabolic perturbations on ratios and overall levels of cofactors used by epigenetic modifiers. Sources for perturbation data: Ac-CoA/CoA, liver levels in fed/fasted rats;⁶⁰ α-KG/R-2HG, tumor levels in IDH1 R132 wild type/mutant tumors;⁴⁴ SAM/SAH, cell lines in control/NNMT overexpressing cell lines.⁶³ α-KG/R-2HG levels were calculated as the average of wild type/mutant samples from reference. All data are provided as unit-less ratios and fold-change to account for differences in units of measurement from each study, and have been rounded to the nearest decimal for simplicity.

Epigenetic modifier	Regulatory metabolite	Perturbation	Native ratio	Perturbed ratio	Fold change - metabolite
KAT	Ac-CoA	fasting	1.2	0.7	1.1
KAT	CoA	fasting	1.2	0.7	1.9
KDM	a-KG	IDH1 mutation	0.2	0.002	0.9
KDM	R-2-HG	IDH1 mutation	0.2	0.002	64.9
PMT	SAM	NNMT overexpression	1.2	0.2	1.9
PMT	SAH	NNMT overexpression	1.2	0.2	0.4