Skip to main content
. Author manuscript; available in PMC: 2014 Feb 9.
Published in final edited form as: Mol Reprod Dev. 2011 Jan;78(1):33–47. doi: 10.1002/mrd.21264

Fig. 3.

Fig. 3

FER kinase is localized within the GV during meiotic arrest. Oocytes were collected from PMSG primed ovaries into FHM+cilostamide to maintain GV arrest. Incompetent NSN oocytes exhibited FER sequestered primarily within the GV (A). Competent SN oocytes also contain high levels of FER kinase located primarily within the GV (B), although they also had an increased level of FER in the cytoplasm as compared to the NSN oocytes. By 30 minutes of maturation, GV levels of FER were further reduced (C). The FER within the GV localize near to but no overlaying chromatin. (Green = FER; red = f-actin; white = DNA. Bar = 10 μm. A, B & C are confocal sections (2 μm thick) taken through the center of the chromatin and showing the entire oocyte. A’, B’ & C’ enlargements of the nuclear region within the same oocytes. A”, B” & C” are the same images as A, B & C but with red (actin) included to better show the outline of the oocytes and the actin matrix surrounding the chromatin (A” & B”).