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. Author manuscript; available in PMC: 2014 Dec 19.
Published in final edited form as: Cell. 2013 Dec 19;155(7):1479–1491. doi: 10.1016/j.cell.2013.12.001

Figure 5. Tracking of telomere dynamics in live cells by CRISPR imaging.

Figure 5

(A) CRISPR imaging of telomeres in RPE cells (scale bar: 5 μm) and trajectories of three telomeres with different movement modes (scale bars: 200 nm). The trajectory lengths are 600 frames for 1 & 3 and 260 frames for 2. See Movie S1. (B) Comparison of telomere dynamics using CRISPR (blue) and EGFP-TRF1 (red) labeling in RPE cells. The data are displayed as mean ± standard error. (C) Scatter plot of the CRISPR foci intensity and their microscopic diffusion coefficients. (D) The average MSD curves of telomeres in UMUC3 cells without (blue) and with (orange) hTR. The data are displayed as mean ± standard error. (E) Averaged MSD curves of CRISPR-labeled telomeres in RPE cells measured with scrambled shRNA (blue), TIN2 shRNA (green), or co-expression of TIN2 shRNA and the long (L, red) or short (S, purple) isoform of TIN2. At least 15 cells are analyzed in each case. The data are displayed as mean ± standard error. See also Figure S5 and Movie S1.