Figure 3.

Electrophysiological responses of single Cajal-Retzius cells to electrical stimulation: (A) Voltage traces illustrating the typical response of a Cajal-Retzius cell upon injection of de- and hyper-polarizing current pulses. (B) Five consecutive current-traces illustrating typical inward current evoked by electrical stimulation in a tangential slice. (C) The stimulus-evoked inward current was abolished in the presence of 1 µM TTX (magenta trace). (D) Two typical current traces recorded from the same trial illustrating that the stimulus-induced inward current is unaffected in the presence of 50 µM D-AP5 and 10 µM CNQX (purple trace). (E) The stimulus-induced inward current was reduced in the presence of 1 µM STR (green trace) and was abolished when both GABA_A and glycine receptors were blocked with 50 µM STR and 50 µM PTX, respectively (red trace). (F) The stimulus-induced inward current was reduced in the presence of 50 µM PTX (blue trace). (G) Statistical analysis. Bars represent the mean ± SEM, numbers of experiments are given in the bars.