Table 1.

Summary of studies transplanting adult SVZ-derived cells into the adult striatum.

Study	Type of SVZ tissue transplanted	Donor species and genotype	Modifications to donor SVZ tissue prior to transplantation	Recipient species and phenotype	Analysis of recipient animals post-transplantation	Key findings
Lois and Alvarez-Buylla, 1994	~100 μm diameter explants	NSE-LacZ mice	None	Wild-type mice	30 days	No migration of surviving cells outside of transplantation site
Herrera et al., 1999	~150 μm diameter explants	NSE-LacZ mice	None	Mice with kainic acid lesions	2, 4, 6, and 8 weeks	Limited migration of transplanted cells Vast majority of surviving NSE-LacZ expressing cells were astrocytes
Zhang et al., 2003	Dissociated cell suspensions	Wild-type rat	Neural progenitor cells cultured for 8 days in media with FGF2, horse and calf serum; cells were labeled with either BrdU or DiO	Wild-type rats	7 and 28 days	Substantial migration away from transplantation site Majority of BrdU and DiO labeled cells expressed either MAP2 or NeuN, few expressed GFAP
Meissner et al., 2005	Neurosphere suspensions	βActin-CMV-GFP mice	Neurospheres were cultured for 5 passages in the presence of EGF and FGF2	Mice with unilateral 6-hydroxydopamine OHDA lesions	1 month	Low survival rate of transplanted cells ~7% and ~2% of surviving cells expressed TH Improved motor performance to apomorphine and amphetamine challenges
Richardson et al., 2005	Dissociated cell suspensions	Wild-type rat	Neural progenitor cells isolated by a Percoll gradient, cultured for 1 week in media containing FGF2, then 6 days in media containing retinoic acid without FGF2; cells were labeled with BrdU	Rats with unilateral 6-hydroxydopamine OHDA lesions	2 weeks	Many surviving transplanted cells maintain Nestin expression, but none expressed NeuN Improved motor performance to amphetamine challenge
Seidenfaden et al., 2006	~100 μm diameter explants	βActin-CMV-GFP mice	None	Wild-type mice	3 weeks and 6 months	Limited migration and low survival rate of transplanted cells Most surviving cells expressed glial marker genes
Chen et al., 2007b	Neurosphere suspensions	Wild-type rat	Neural progenitor cells isolated by a Percoll gradient protocol, cultured for 2 weeks in media containing EGF and FGF2; cells were labeled with BrdU	Wild-type rat	6 weeks	Substantial migration away from transplantation site Most surviving cells expressed GFAP and none expressed NeuN
Chen et al., 2007a	Neurosphere suspensions	Wild-type rat	Neural progenitor cells isolated by a Percoll gradient protocol, cultured for 2 weeks in media containing EGF and FGF2; cells were labeled with BrdU	Rats transduced with AAV1/2 vectors to over-express either BDNF or FGF2 in the striatum	6 and 12 weeks	AAV1/2-FGF2 improved survival of transplanted cells Most surviving transplanted cells expressed glial marker genes AAV1/2-BDNF increased percentage of surviving cells expressing NeuN
Shim et al., 2007	Dissociated cell suspensions	Wild-type rat	Neurospheres were cultured in media containing EGF, FGF2, and PDGF for over 2 months; retroviral transduction to over-express Nurr1 and Mash1	Rats with unilateral 6-hydroxydopamine OHDA lesions	2, 4, and 6 weeks	Substantial number of surviving transplanted cells expressed MAP2, TH and VMAT2 Improved motor performance to amphetamine challenge
Deleidi et al., 2011	Dissociated cell suspensions	rTA/Oct4 mice	Neurospheres were expanded for 14 days before being converted to induced pluripotent stem cells and then differentiated via 5 stage protocol; SSEA1+ cells were removed by cell sorting prior to transplantation	Rats with unilateral 6-hydroxydopamine OHDA lesions	4, 6, and 8 weeks	Substantial number of surviving transplanted cells express TH Improved motor performance to apomorphine and amphetamine challenges