Figure 6.

Effect of metformin or rapamycin alone and in combination on TPA-induced mTORC1 signaling. Pooled protein lysates were prepared from epidermal scrapings of FVB/N mice (n=5/group) undergoing a multiple treatment regimen of acetone, metformin alone (50 mg/kg), 6.8 nmol of TPA alone, 50 mg/kg metformin+TPA, 2 nmol of rapamycin+TPA, or combinations of these two treatments prior to TPA. A, Representative Western blot analyses of mTORC1 downstream targets p-p70S6K and PDCD4. B, Quantification of Western blots from three independent experiments. Expression levels were normalized to p-actin and averaged. * Indicates significantly different from the TPA-treated group (p<0.05, Mann Whitney U-test). **Indicates significantly different values between the indicated treatment groups (p<0.05, Mann Whitney U-test).