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. 2014 Jan 21;111(5):1951–1956. doi: 10.1073/pnas.1310779111

Fig. 5.

Fig. 5.

Induction of ChREBP and PERK-mediated SREBP1 activation are independent pathways in HCMV-infected cells. (A) Depletion of ChREBP had little effect on the induction of PERK and the mature forms of SREBP1 during HCMV infection. Whole-cell extracts were made at 72 hpi, and protein levels were measured by Western blot analysis. The M-SREBP1 blot was spliced to match the order of other blots. (B and C) Depletion of PERK had little effect on the induction of ChREBP and GLUT4 during HCMV infection. (B) RNA levels of total ChREBP and GLUT4 in shLuc- and shPERK-treated cells. (C) Protein levels of ChREBP-α, GLUT2, and GLUT4 in PERK-depleted cells. M, mock infection; V, HCMV infection; M-SREBP1, mature forms of SREBP1.