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. 2014 Jan 21;111(5):1861–1866. doi: 10.1073/pnas.1309915111

Fig. 3.

Fig. 3.

All known heme-binding–deficient point mutants of DGCR8 are defective in pri-miRNA processing. HeLa cells were cotransfected with the pri-miR-9-1 reporter and N-flag-DGCR8 expression plasmids as indicated. (A) The normalized eYFP/mCherry slope (± 95% CI) of N-flag-DGCR8 C352A. (B and C) qRT-PCR analyses (mean ± SD, n = 3) of the (B) mCherry–pri-miRNA fusion RNA and (C) the mature miR-9 of the cotransfections in A. (D and E) Anti-flag immunoblots with equal amounts of nuclear extracts loaded. (F) Normalized fluorescence slopes (± 95% CI) for transfections with the DGCR8 mutants. (G and H) qRT-PCR analyses of (G) mCherry–pri-miRNA fusion RNA and (H) mature miR-9 for the transfections in F. Error bars represent SD (n = 3). P values are labeled on the graphs. The eYFP vs. mCherry fluorescence scatter plots are shown in Fig. S6 A–D.