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. 2014 Jan 21;111(5):1843–1848. doi: 10.1073/pnas.1323416111

Fig. 2.

Fig. 2.

Fewer spermatids, reduced spermatogonial proliferation, and increased apoptosis and DNA damage in TAp73 KO testes. (A–C) Representative immunohistochemical analysis to detect TAp73 (A), protamine 1 (B), and Ki67+ (C) in testes of 36-wk-old WT and TAp73 KO mice. Arrowheads in A show positive staining in cytoplasm of spermatogonia. (D) Quantitation of the data in C expressed as percentage of Ki67+ cells. Data shown are the means ± SD (n = 5). (E) Representative TUNEL assay detecting apoptosis in testes of 36-wk-old WT (n = 4) and TAp73 KO (n = 5) mice. (F) Quantitation of the data in E expressed as percentage of TUNEL+ cells. Data shown are the means ± SD. (G) Representative immunohistochemical analysis to detect the DNA damage marker γH2AX in testes of 16-wk-old WT (n = 5) and TAp73 KO (n = 5) mice. (H) Quantitation of the data in G expressed as percentage of γH2AX+ cells. Data shown are the mean ± SD. P values were determined according to unpaired Student t test.