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. 2014 Jan 21;111(5):1843–1848. doi: 10.1073/pnas.1323416111

Fig. 4.

Fig. 4.

MMP13 and ADAM17 are previously unidentified TAp73 target genes. (A) HA tag TAp73β, ΔNp73, and p53 Saos-2 tet-on cells were exposed to 2 μM doxycycline for 16 h, followed by qPCR determinations of the indicated mRNAs. Data shown are the means ± SD (n = 3). P values were determined according to unpaired Student t test. (B) Bioinformatics analysis of human ADAM17 and MMP13 promoters using the MatInspector program has been analyzed for putative p53 binding sites (p53BS). (C) ChIP assay was performed using nuclear extracts from HA-TAp73β–overexpressing Saos-2 cells. Protein–chromatin complexes were immunoprecipitated with anti-HA antibody or control IgG. PCR was performed with primers designed against promoter region predicted or validated p53-binding sites of indicated genes. MDM2- and p21-responsive elements were used as positive controls.