Harvesting pigment cells from zebrafish fins. (A) Adult WT zebrafish. (B) Magnified image of the surface of a WT fish body. Black spots are melanophores, and yellow spots are xanthophores. Clear gaps exist between the regions containing melanophores and the regions containing xanthophores. (C) A schematic diagram describing the harvest of pigment cells from fins. (D and E) The effect of serum in the medium on cell spreading. Cell pellets were suspended in (D) serum-free L15 medium or (E) medium containing 1% FBS. (Scale bars: A, 5.0 mm; B, D, and E, 100 μm.)