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. 2014 Jan 21;111(5):1855–1860. doi: 10.1073/pnas.1323761111

Fig. 2.

Fig. 2.

The β-catenin Tyr142-phosphorylation induced by EC–SMC contact is regulated by Cx43/Fer. ECs were kept as a monoculture or cocultured with SMCs for 6, 18, and 24 h, and the Cx43 expression (A) and Fer phosphorylation (D) were determined by Western blot. ECs were pretreated with a control (Gap27CL) or Cx43 (Gap2743) blocking peptide (B) and with DMSO or RTK inhibitors (C), and transfected with control or Cx43-specific siRNA (B and D) and Fyn- or Fer-specific siRNAs (C), and then cocultured with SMCs for 18 h. β-Catenin (B and C) or Fer (D) phosphorylations were determined by Western blot. The results are the mean ± SEM from three independent experiments. *P < 0.05 vs. monocultured ECs. #P < 0.05 vs. cells pretreated with inhibitor or transfected with control siRNA.