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. 2014 Jan 21;111(5):1855–1860. doi: 10.1073/pnas.1323761111

Fig. 4.

Fig. 4.

The Ser45/Thr41-phosphorylation of β-catenin in ECs induced by SMC-CM is mediated by SMC-released BMPs through BMPRII/Smad5. (A) ECs were pretreated with vehicle or Noggin, and then treated with SMC-CM for 18 h. (B) ECs were kept as a monoculture or cocultured with SMCs for 2, 4, and 12 h or treated with SMC-CM for 1, 6, 12, 18, and 24 h. (C) ECs were transfected with control, BMPRII-, Smad1-, or Smad5-specific siRNA, and then treated with SMC-CM for 18 h. β-catenin (A and C) and Smad1/5 (B) phosphorylations were determined by Western blot. The VE-cadherin–associated β-catenin Ser45/Thr41 phosphorylation was determined by immunoprecipitation and Western blot (D). The results are the mean ± SEM from three independent experiments. *P < 0.05 vs. monocultured or unstimulated control cells; #P < 0.05 vs. cells pretreated with vehicle or transfected with control siRNA.