Fig. 1.

Subunit arrangement in the CLRC complex. (A) Testing of pairwise interactions between differently tagged CLRC subunits after coexpression and pull-down using the OneSTrEP SumoStar (OSS)-tagged protein as bait. Boxes group different classes of interaction results (new: red, lower confidence; new: pink, previously reported: blue, no interaction: black) (B) Matrix summarizing all pull-downs performed in this work color coded as in A. (C) Schematic diagram showing the subunit arrangement in the CLRC complex based on the results of the pairwise interaction screen. The arrow indicates the putative target recognition surface of Dos1 (see below). (D) The structure of the CLR4^DDB2 complex (PDB ID code 4A0K). Equivalent proteins of the CRL4 and CLRC complexes are shown in similar colors.