Fig. 5.

The putative target-binding surface of Dos1 is important for heterochromatic silencing. (A) Representative results from comparative growth assays of the serially diluted dos1 null strain with the centromeric otr1R::ura4 reporter expressing Dos1 mutants as indicated from a plasmid. Strains were examined for growth on a PMG medium lacking leucine and supplemented with 5-FOA (+FOA –Leu), PMG medium lacking uracil and leucine (−Ura −Leu), and PMG medium lacking leucine (–Leu). Cells were always grown on PMG medium lacking leucine to select for Dos1-expressing plasmid (SI Appendix, Table S1). (B) Surface representation of Dos1WD mapped mutations colored by the degree of the silencing defect (red: severe defect; orange: less severe defect; yellow: mild defect; light yellow: very mild defect in silencing; blue: no effect in ura4⁺ silencing).