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. 2014 Jan 21;111(5):1963–1968. doi: 10.1073/pnas.1316901111

Fig. 3.

Fig. 3.

PhoPQ activation increases the levels of CL and palmitoyl-PG species within the OM. (A) ESI-MS of OM CL. ψSpecies was quantified by LC-MS/MS (Table S2). (B) The average fold difference ± SD for all CL/PE comparisons from four experiments. ωThe average pg/ng value is significantly greater than wild type by an unpaired Student t test. Unpaired t tests were used throughout the study where significance is indicated. (C) ESI-MS of IM and OM acyl-PG. *Denotes a palmitoyl-PG (21). ξm/z 983.9 reflects two distinct acyl-PG species. (D) LC-MS/MS determined the acyl-PG/ PE (pg/ng) in OM extracts (Table S3). Shown is the average value from three experiments ± SD ωThe value is significantly different from wild type. (E) S. Typhimurium was treated with polymyxin B (PMB), and acyl-PG species were quantified by LC-MS/MS. The average value from two experiments ± SD is shown alongside the values obtained for mutant bacteria (Table S3).