Fig. 1.

Design of PGM1 and PGM2 mutant mPolκ. (A) A back view of the structure of hPolκ–DNA–dNTP complex structure (PDB ID code 2OH2). Polκ is shown in gold ribbon diagram and covered with a semitransparent molecular surface. The residues to be mutated are shown in ball-and-stick model. The DNA template strand is shown in magenta and the template base is highlighted in red opposite the incoming dNTP (cyan). (B) Structure of Dpo4–DNA–dNTP complex (PDB ID code 2AGQ). After superposition with Polκ, Dpo4 is shown in blue with the corresponding residues along the structural gap highlighted in ball-and-stick models. (C) Models of PGM1 and (D) PGM2 mutant mPolκ. The mutated residues are highlighted in blue and labeled according to mPolκ residue number. The corresponding human residues are exactly one number higher (Fig. S1).