FIGURE 2.

TSLP-DCs induce expansion of CD4⁺CD25⁺FOXP3⁺ T cells from decidual CD4⁺CD25⁻ T cells in vitro. (A) Purified CD4⁺CD25⁻ T cells were incubated with DCs pretreated with PBS, rhIL-7, rhTSLP, or TCM or with TCM combined with αTSLP-DCs at a 1:6 ratio of DCs to T cells for 3 or 7 d. Cells were analyzed by FCM gating of CD4⁺ cells. (A) Percentages of CD4⁺CD25⁺FOXP3⁺ T cells are indicated. (B) Representative FCM data after 7 d of culture are presented. (C) Percentages of CD4⁺CD25⁺FOXP3⁺ T cells determined by FCM following 7-d coculture of decidual CD4⁺CD25⁻ T cells with or without rhTSLP-DCs in the presence or absence of anti–TGF-β1 or anti–IL-10 neutralizing Abs or isotype control. Results are shown from three independent experiments with three different deciduas and 17 villi. Data are shown as mean ± SE. A representative graph is shown. *p < 0.05 and **p < 0.01 compared with control; ^△△p < 0.01 compared with rhTSLP-DC. Two-way ANOVA with Bonferroni posttests was used in (C). αIL-10, neutralizing Ab against IL-10; αTGF-β1, neutralizing Ab against TGF-β1; Ctrl, control.