FIGURE 5.

TSLP-dDC–induced decidual CD4⁺CD25⁺FOXP3⁺ T cells produce Th2 cytokines. Decidual CD4⁺FOXP3⁺ T cells generated by TSLP-DC were processed for FCM analysis after staining for IL-4 or IL-5, CD4, FOXP3, and GATA3. Decidual CD4⁺CD25⁻ T cells were cultured with TSLP-dDCs for 7 d. Cultured cells were then stimulated with PMA and ionomycin. Expression levels of cell surface CD4 and intracellular IL-4, IL-5, FOXP3, and GATA3 at the single-cell level were analyzed by FCM. In parallel experiments, the polarizations of Th1 and Th2 were induced as the positive and negative controls for the positivity gates identifying GATA3⁺ cells, IL-5⁺ cells, and IL-4⁺ cells. Results are shown from three independent experiments with three different deciduas. The dot plots are representative of three experiments with three different deciduas.