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. Author manuscript; available in PMC: 2014 Feb 10.
Published in final edited form as: Biochem Pharmacol. 2010 Jul 15;80(12):2057–2065. doi: 10.1016/j.bcp.2010.07.003

Figure 6. Resveratrol decreases the expression of a SMAD2/SMAD3/SMAD4 luciferase reporter contruct.

Figure 6

After cotransfection with the SMAD2/SMAD3/SMAD4 luciferase reporter plasmid along with either a Control RNA (pre-miR-Control) or a miR-663 antisense inhibitory RNA (663-I), SW480 cells were mock-treated or treated with TGFβ for 14 hours, and subsequently treated with the vehicle (Veh.) or resveratrol (Res.) for another 14 hours. The Firefly luciferase activity was measured 48 hours after transfection and then normalized to the Renilla luciferase activity. Values represent the mean ± standard deviation (n = 6). * and **, TGFβ1-treated significantly different from the corresponding Mock-treated, *, P < 0.03, **, P < 0.002; # and ##, Resveratrol-treated significantly different from the corresponding Vehicle-treated, #, P < 0.03, ##, P < 0.005 (df = 10).