Figure 4. Steady-state kinetic assay and catalytic characteristics of the palladium nanostructures toward various components: (A,C) 0.5 mM TMB and different-concentration H₂O₂, (B,D) 500 mM H₂O₂ and different-concentration TMB, and (E,F) different-concentration TMB and different-concentration H₂O₂.

The initial rate (v) of the reaction was measured using 40 μL of the prepared palladium nanostructures in 400 μL of sodium citrate-phosphate buffer solution (pH 4.0) for 5 min at room temperature. Error bars indicate standard deviations (n = 3).