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. 2014 Feb 10;4:3993. doi: 10.1038/srep03993

Figure 1. The kinetics of variant specific T cell activation following viral reactivation in a seronegative transplant recipient.

Figure 1

(a) PBMC from a transplant patient (Tx1) at different stages post-transplant were co-stained with a PE-conjugated HLA B8/ELK_IYM dextramer and an APC conjugated HLA B8/ELR_MYM dextramer, then labeled with anti-CD8. (b) PBMC from Tx1 were incubated for five hours with the HLA B8-restricted IE-1 encoded peptide epitope variants, labeled with anti-CD8 and then assessed for intracellular expression of IFN-γ. Data shows the percentage of IFN-γ producing CD8+ T cells reactive against each of the epitope variants. (c) Following in vitro expansion for two weeks in the presence of cognate peptide and IL-2, ELR_MYM or ELK_IYM specific T cells were incubated for four hours with all four peptide variants, then IFN-γ expression was assessed using an intracellular cytokine assay. FACS plots show the proportion of ELR_MYM or ELK_IYM expanded CD8+ T cells responding to the peptide epitope variants.