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. Author manuscript; available in PMC: 2014 Feb 10.
Published in final edited form as: Methods Mol Biol. 2012;845:19–39. doi: 10.1007/978-1-61779-539-8_2

Figure 1.

Figure 1

PCR amplification of the mini blaster cassette from the pDDB57 plasmid. Grey boxes in the diagram indicate the 200 bp repeats flanking the URA3 ORF. In the gel picture, lanes 1 to 5 show the ~2000 bp long cassette and a ~750 bp long loop-out product.