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. Author manuscript; available in PMC: 2014 Feb 10.
Published in final edited form as: Methods Mol Biol. 2012;845:19–39. doi: 10.1007/978-1-61779-539-8_2

Figure 4.

Figure 4

Colony PCR based detection for putative homozygous mutants indicating the positions of detect primers (FD2 and RD1). Lanes 1 to 7 show PCR analysis on independent colonies. Two possible situations indicated by desired and alternative outcome are shown in the diagram. In the gel picture, lanes marked 4 and 7 indicate an expected outcome situation where the previously undisrupted allele is replaced by the cassette leading to loss of a wild type detect band (refer to the positive control lane).