| 10X PCR buffer | 5 μL |
| 5 mM dNTPs | 4 μL |
| 10 μM forward primer (F1) | 1 μL |
| 10 μM reverse primer (R1) | 1 μL |
| Takara Ex Taq™ Polymerase | 0.5 μL |
| pDDB57 template (1:50) | 1 μL |
| dH20 | 37.5 μL |
|
| |
| Total Volume | 50 μL |
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