| Extracted plasmid DNA | 3 μL |
| 10X NE Buffer 3 | 2 μL |
| 100X BSA | 0.2 μL |
| NotI | 0.2 μL |
| EcoRI | 0.2 μL |
| dH2O | 14.4 μL |
|
| |
| Total Volume | 20 μL |
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