| Component | Volume (μl) |
|---|---|
| Milli-Q water | 38.5 |
| Pfu buffer (10×) | 5 |
| dNTP (10 mM) | 2 |
| TruSeq PCR primer cocktail | 1.5 |
| Bisulfite-converted DNA | 2 |
| Pfu Turbo Cx DNA polymerase | 1 |
| Total | 50 |
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