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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Protoc. 2013 Sep 5;8(10):1841–1851. doi: 10.1038/nprot.2013.115
Component Volume (μl)
Milli-Q water 5.5
Designed forward primer (10 μM) 2.5
Designed reverse primer (10 μM) 2.5
Bisulfite-converted DNA 2
KAPA HiFi uracil+ master mix (2×) 12.5
Total 25