Component | Volume (μl) |
---|---|
Milli-Q water | 5.5 |
Designed forward primer (10 μM) | 2.5 |
Designed reverse primer (10 μM) | 2.5 |
Bisulfite-converted DNA | 2 |
KAPA HiFi uracil+ master mix (2×) | 12.5 |
Total | 25 |
Component | Volume (μl) |
---|---|
Milli-Q water | 5.5 |
Designed forward primer (10 μM) | 2.5 |
Designed reverse primer (10 μM) | 2.5 |
Bisulfite-converted DNA | 2 |
KAPA HiFi uracil+ master mix (2×) | 12.5 |
Total | 25 |