Component	Digestion control (volume)	Digestion negative control (volume)	Cutting control (volume)
Taq1 buffer (10×)	2 μl	2 μl	2 μl
Taq1 RE (20 U μl−1)	40 U	—	40 U
BSA	0.2 μl	0.2 μl	0.2 μl
Processed digestion control  amplicon DNA (10 ng μl−1)	10 μl	10 μl	—
Cutting control DNA (20 ng μl−1)	—	—	5 μl
Milli-Q water	5.8 μl	7.8 μl	10.8 μl
Total	20 μl	20 μl	20 μl