Skip to main content
. Author manuscript; available in PMC: 2014 Feb 10.
Published in final edited form as: Exp Cell Res. 2007 Feb 22;313(8):1652–1666. doi: 10.1016/j.yexcr.2007.02.008

Fig. 2. Levels of Tcf associated beta-catenin in mock and NaB treated CRC cells.

Fig. 2

(A,B) Representative Western blot analyses of beta-catenin co-immunoprecipitated with Tcf4 antibody as described in Materials and Methods. Equal amounts of immunoprecipitated samples were separated by SDS-PAGE, transferred to nitrocellulose membranes, and immunostained with antibody to beta-catenin (Transduction Laboratories). (C) Representative Western blot analyses of the steady-state levels of Ser-37/Thr-41-dephosphorylated beta-catenin and Tcf4 in the nuclear lysates used for the immunoprecipitation experiments. Equal loading was confirmed by staining the unused portion of the membranes with Ponceau's solution.