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. Author manuscript; available in PMC: 2014 Dec 26.
Published in final edited form as: Virus Res. 2013 Sep 17;178(2):398–403. doi: 10.1016/j.virusres.2013.09.013

Table 1.

Protection against H5N1 virus challenge.

Group Vector/s Vector dose Challenge on Day Post-inoculation Lung virus titer (mean±SD)
1 HAd-HA-NP 2 × 107 Day 7 2.53±0.33*
2 HAd-HA-NP+HAd-Mbd2 2 × 107 + 1 × 107 Day 7 ≤0.50
3 HAd-HA-NP 1 × 108 Day 7 2.25± 0.16*
4 HAd-HA-NP + HAd-Mbd2 1 × 108 + 1 ×107 Day 7 ≤0.50
5 HAd-HA-NP 5 × 108 Day 7 ≤0.50
6 HAd-HA-NP+ HAd-Mbd2 5 × 108 + 1 ×107 Day 7 ≤0.50
7 HAdΔE1E3 + HA-Mbd2 1 × 108 + 1 ×107 Day 7 4.39±0.30
8 HAdΔE1E3 1 × 108 Day 7 4.55±0.46
9 HAd-HA-NP 2 × 107 Day 14 ≤0.50
10 HAd-HA-NP + HAd-Mbd2 2 ×107 + 1 ×107 Day 14 ≤0.50
11 HAd-HA-NP 1 × 108 Day 14 ≤0.50
12 HAd-HA-NP + HAd-Mbd2 1 × 108 + 1 ×107 Day 14 ≤0.50
13 HAd-HA-NP 5 × 108 Day 14 ≤0.50
14 HAd-HA-NP+HAd-Mbd2 5 × 108 + 1 ×107 Day 14 ≤0.50
15 HAdΔE1E3 + HA-Mbd2 1 × 108 + 1 ×107 Day 14 4.40±0.28
16 HAdΔE1E3 1 × 108 Day 14 4.32±0.07

Mouse β-defensin 2 (Mbd2) enhances the protective efficacy of HAd-HA-NP vaccine against H5N1 influenza virus challenge. Groups of mice (N=10) were intramuscularly vaccinated with 2 × 107, 1 × 108, or 5 × 108 plaque forming units (pfu) of HAd-HA-NP with or without 1 × 107 pfu of HAd-Mbd2. Animals that similarly received 1 × 108 pfu of the HAd-ΔE1E3 or 1 × 108 pfu of HAd-ΔE1E3 + 1 × 107 pfu of HAd-Mbd2 served as controls. At Days 7 or 14 post-immunization, five animals/group were challenged intranasally with a 100 50% mouse infectious dose (MID50) of a reverse genetics derived A/Puerto Rico/8/1934(H1N1) [PR8] virus containing hemagglutinin (HA) and neuraminidase (NA) gene fragments of A/Vietnam/1203/04 (H5N1) [VNH5N1-PR8/CDC-RG]. Day 3 after challenge, the animals were euthanized, and the lung virus titers were determined by 50% tissue culture infective dose (TCID50) analysis on MDCK cells to evaluate the protective efficacy of the vaccine. The data represent the mean virus titers ± standard deviation (SD) from five animals per group. The detection limit of the lung viral titer was 0.5 Log10 TCID50/ml (indicated as <0.50).

*

P≤0.05 compared to mice vaccinated with a similar dose of HAd-ΔE1E3.