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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: J Mol Biol. 2013 Oct 23;426(3):510–525. doi: 10.1016/j.jmb.2013.09.043

Figure 2.

Figure 2

Wild type Vps4p remains hexameric and Vps4p(E233Q) remains dodecameric in the presence of Vta1p. (A) ATP-bound wild-type and E233Q Vps4p size exclusion chromatograms. Calculated retention volumes for 6:6, 6:12, and 12:12 Vps4p:Vta1p complexes are indicated. The void volume and the expected elution volume for the Vps4p hexamer are indicated by arrows. (B) SDS PAGE analysis of the peak fraction of wild-type Vps4p (48 kDa) in the presence of Vta1p (37 kDa) confirms that the peak represents a complex of both proteins.