Figure 3. Ventralization of asun^d93-derived eggs.

(A) Eggs laid by asun^d93 females. Anterior, top; dorsal side facing outward. Classification scheme for ventralized eggs is adapted from (Lei and Warrior, 2000). Class I eggs appear wild type with a pair of dorsal appendages in the anterior-dorsal region. Dorsal appendages in class II eggs are positioned abnormally close to each other. Class III and class IV eggs contain dorsal appendages that are partially fused at the base and completely fused along the length, respectively. Class V eggs lack dorsal appendages. Scale bar, 250 μm. (B) Quantification of dorsal appendage phenotypes in embryos from wild-type, asun^d93, and rescued asun^d93 females (>200 embryos scored per genotype).