Figure 4. Localization of grk transcripts and Grk protein in asun^d93 oocytes.

(A–B) Fluorescent in situ hybridization of stage 9 egg chambers using grk probe (red). Dorsal, top; anterior, left. grk mRNA localization is tightly restricted to the anterior-dorsal region of the oocyte in wild-type egg chambers (A). In asun^d93 egg chambers, grk transcripts are more diffusely localized throughout the anterior oocyte (B). Scale bars, 50 μm. (C) Quantification of abnormal gurken mRNA localization in wild-type, asun^d93, and rescued asun^d93 egg chambers (>100 chambers scored per genotype) by fluorescent in situ hybridization. Asterisks, p<0.0001. (D–E″) Maximum projection confocal images of stage 10 wild-type and asun^d93 egg chambers stained with Gurken (red; grayscale in D″ and E″) and F-Actin (green; cell membrane marker). Anterior, left; dorsal, top. Gurken protein localizes normally to the anterior-dorsal region of asun^d93 oocytes (E, E′, E″), but occasionally with a lower intensity and more diffuse pattern than that observed in wild-type oocytes (D, D′, D″). Scale bars, 20 μm.